Secretory expression and biological function of IGFBP-3 in eukaryotic cells / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 558-562, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-342871
ABSTRACT
The cDNA of Insulin-like growth factor binding protein 3 was subcloned into a eukaryotic secretory expression vector pSectagA to construct pSectag-IGFBP3. Human renal cell carcinoma (RCC) 786-0 cells were transfected with pSectag-IGFBP3 using lipofectamine 2000. After 48 h, the secretory IGFBP-3 was tested and identified by western blotting. Meanwhile, Annexin V-EGFP stain was used to analyze the apoptosis of 786-0 cells induced by IGFBP-3. Secretory IGFBP-3 protein could express successfully in the 786-0 cells and the expressed IGFBP-3 directly displayed an apoptotic effect on the host cells. This work provides a basis for further study on the apoptosis-inducing mechanism of IGFBP-3 and the development of a new anti-tumor drug.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Physiologie
/
Protéines de fusion recombinantes
/
Données de séquences moléculaires
/
Cellules cancéreuses en culture
/
Séquence nucléotidique
/
Néphrocarcinome
/
Transfection
/
Apoptose
/
Protéine-3 de liaison aux IGF
Type d'étude:
Étude pronostique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2008
Type:
Article
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