Generation of mouse UBE2W antibody and analysis of UBE2W expression in mouse tissues / 生物工程学报
Chinese Journal of Biotechnology
; (12): 547-552, 2008.
Article
Dans Zh
| WPRIM
| ID: wpr-342873
Responsable en Bibliothèque :
WPRO
ABSTRACT
Ubiquitin conjugating enzyme functions as the second enzyme required for protein ubiquitination and plays an important role in ubiquitin transferring and substrate specific recognition. UBE2W, a newly described member of E2 family, was formerly reported probably involving in phototransduction or retinal degeneration in Drosophila. In this study, we report that murine UBE2W harbors a typical UBC domain and is highly conserved in different vertebrate homologues. GST-tagged UBE2W was expressed in E. coli BL21 (DE3) and purified with GST affinity chromatography. Using this antigen, we generated and further separated rabbit polyclonal antibody of UBE2W, of which the activity and specificity were confirmed by immunoblotting of transiently expressed myc-UBE2W fusion protein. Wide expression of UBE2W was found in brain, muscle, heart, lung, liver, spleen, kidney and testis of mouse with the generated antibody, indicating the functional importance of this novel protein. Furthermore, the UBE2W highly expression was confined to the adult testis and was developmental stage-specific.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Testicule
/
Protéines de fusion recombinantes
/
Distribution tissulaire
/
Ubiquitin-conjugating enzymes
/
Allergie et immunologie
/
Escherichia coli
/
Génétique
/
Métabolisme
/
Anticorps
/
Anticorps monoclonaux
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Chinese Journal of Biotechnology
Année:
2008
Type:
Article