p16 and MGMT gene methylation in sputum cells of uranium workers / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
;
(12): 92-95, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-343054
ABSTRACT
<p><b>OBJECTIVE</b>To study the methylation of O-6-methylguanine-DNA methyltransferase (MGMT) and p16 gene in the sputum cells of radon-exposed population. To provide the experimental base for finding the molecular biomarker of the high risk population of the radon-induced lung cancer.</p><p><b>METHODS</b>91 radon-exposed workers were divided into 4 groups, high dosage group (> 120 WLM), middle dosage group (between 60 and 120 WLM), low dosage group (between 30 and 60 WLB) and lower dosage group (between 2 and 30 WLM) according to the accumulated exposure dosage of the radon daughters. The abnormal methylation of p16 and MGMT gene in the sputum cells of the population in the four groups was detected with the methylation specific PCR (MSP).</p><p><b>RESULTS</b>There was significantly upward trend for the p16 gene methylation rate (0.00%-20.00%), the MGMT gene methylation rate (0.00%-28.00%) and the total methylation rate (0.00%-40.00%) with the increase of the accumulated exposure dosage of the radon daughters (P < 0.01).</p><p><b>CONCLUSION</b>The methylation of p16 and MGMT gene is related to the accumulate exposure dosage of the radon daughters.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Expectoration
/
Cancérogènes environnementaux
/
Exposition professionnelle
/
Radon
/
Produits de filiation du radon
/
Méthylation de l'ADN
/
O(6)-methylguanine-DNA methyltransferase
/
Inhibiteur p16 de kinase cycline-dépendante
/
Relation dose-effet des rayonnements
/
Génétique
Limites du sujet:
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Année:
2006
Type:
Article
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