Fluorescence in situ hybridization on bone marrow smear in the detection of cytogenetic aberrations of multiple myeloma / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1230-1233, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-343313
ABSTRACT
This study was aimed to establish the technique of interphase fluorescence in situ hybridization (I-FISH) used on smear of bone marrow directly, and to develop a new method for detection of the molecular cytogenetics in multiple myeloma (MM). After a series of treatment, fixation and digestion of the bone marrow smear as the carrier, the chromosome 8 centromere probe were used in I-FISH for molecular cytogenetics detection. At the same time, differences were compared in the results between the new method and the conventional I-FISH. The results showed that there was no statistically significant difference of proportion of various signals in non-hematologic malignancies when detected with the two methods (p>0.05). In bone marrow smear I-FISH, 8 out of 19 cases (42.1%) had abnormality of chromosome 8, including 5 cases with -8 (26.3%) and 3 cases with +8 (15.8%). It is concluded that the I-FISH on smear of bone marrow is characterized by convenience, economy and accuracy. Therefore, it can be used for research of molecular cytogenetics in MM.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Moelle osseuse
/
Chromosomes humains de la paire 8
/
Aberrations des chromosomes
/
Hybridation fluorescente in situ
/
Génétique
/
Méthodes
/
Myélome multiple
Type d'étude:
Etude diagnostique
Limites du sujet:
Adulte
/
Adulte très âgé
/
Femelle
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Journal of Experimental Hematology
Année:
2009
Type:
Article
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