Inhibitory effects of ginkgolide B on proliferation of bovine aortic smooth muscle cells / 药学学报
Acta Pharmaceutica Sinica
; (12): 90-93, 2002.
Article
de Zh
| WPRIM
| ID: wpr-343394
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>AIM</b>To investigate the effects of ginkgolide B (GB) on proliferation of bovine aortic smooth muscle cells (SMC) and its related mechanisms.</p><p><b>METHODS</b>After pretreating with GB or the mixture of ginkgolide A and B (GA:GB) at 37 degrees C for 0.5 h, the VSMC were treated with or without angiotensin II (Ang II) for 24 h. The proliferation of SMC was evaluated by 3H-thymidine incorporation and the cell cycle phase was measured by flow cytometry. Cytotoxicity was reflected by MTT and lactate dehydrogenase (LDH) activity of the supernatant.</p><p><b>RESULTS</b>Whether or not treated with Ang II, GB and GA:GB were shown to suppress SMC proliferation in concentration-dependent fashion at concentrations ranging from 10(-9) mol.L-1 to 10(-5) mol.L-1. The inhibitory effects appeared to be related to a G1-->S block in cell cycle traverse.</p><p><b>CONCLUSION</b>The suppression of SMC proliferation by GB might not only be contributed by blockage of the PAF receptor activity.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Aorte
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Pharmacologie
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ADN
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Glycoprotéines de membrane plaquettaire
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Division cellulaire
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Cellules cultivées
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Phase G1
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Biologie cellulaire
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Myocytes du muscle lisse
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Récepteurs couplés aux protéines G
Limites du sujet:
Animals
langue:
Zh
Texte intégral:
Acta Pharmaceutica Sinica
Année:
2002
Type:
Article