Effects of botulinum toxin type A on the expression of alpha-SMA and myosin-II of fibroblasts in scars / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 118-121, 2014.
Article
Dans Chinois
| WPRIM
| ID: wpr-343467
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of botulinum toxin type A (BTXA) on the expression of alpha smooth muscle actin(alpha-SMA) and myosin-II of fibroblasts in scars. Methods Fibroblasts were isolated from tissue specimens of scars contracture. Cells from passages 3-5 were randomly divided into 3 groups (control group, low BTXA group (1 U/10(6) Cells), and high BTXA group (2.5 U/ 10(6)Cells)). Growth condition of fibroblasts was observed at 1 , 4, 7 day after BTXA treated. Changes of alpha-SMA and myosin-II in fibroblasts were detected by Western blot.</p><p><b>RESULTS</b>Fibroblasts grew well in control group. The proliferation was decreased 4 days later in BTXA groups. Lots of apoptotic cells were seen in high BTXA group at 7th day. Proteins of alpha-SMA and myosin-II in fibroblasts were statistically different between BTXA group and control groups at 4th day (P < 0.05). The expression of alpha-SMA and myosin-II in low BTXA group was higher than that in high BTXA group at 7th day (P < 0.05).</p><p><b>CONCLUSIONS</b>BTXA could induce the apoptosis of fibroblasts and decrease the expression of alpha-SMA and myosin-II in fibroblasts. The inhibitory effect was strengthened with BTXA concentration increase within a certain range.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Répartition aléatoire
/
Actines
/
Cicatrice
/
Toxines botuliniques de type A
/
Myosine de type II
/
Fibroblastes
/
Métabolisme
/
Muscles lisses
Type d'étude:
Essai clinique contrôlé
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Plastic Surgery
Année:
2014
Type:
Article
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