Application of flow cytometry to the evaluation of semen quality / 中华男科学杂志
National Journal of Andrology
;
(12): 608-611, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-343562
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the role of flow cytometry in semen assessment.</p><p><b>METHODS</b>Semen samples from 104 infertile male patients (as the case group) and 10 fertilized donors (as the control group) were analyzed for the volume of ejaculate and sperm concentration, motility and atypical morphology by computer-assisted semen analysis (CASA), the viability, chromatin structure and mitochondrial membrane potential (MMP) of the sperm stained by SYBR-14/PI, AO and JC-1 respectively, and assessed with flow cytometry. The results were analyzed through SAS software.</p><p><b>RESULTS</b>U tests indicated that the semen from the infertile patients had not only lower concentration (U = 2.51, P = 0.0143), lower motility (U = 3.44, P = 0.001) and higher rate of atypical morphology (U = -5.88, P < 0.0001), but also lower viability (U = 4.72, P < 0.0001), MMP (U = -2.53, P = 0.0309), and chromatin integrity (alpha t U = -3.82, P = 0.0003; SD alpha t U = -3.98, P = 0.0001; COMP alpha t U = -3.57, P = 0.0005). The multiple stepwise regression analysis revealed that sperm motility was positively correlated with sperm membrane integrity (t = 1.66, P = 0.1016), sperm MMP (t = 3.33, P = 0.0014) and sperm acrosome integrity (t = 3.24, P = 0.0019), while sperm MMP was negatively correlated with the rates of sperm neck and tail defects (t = -3.44, P = 0.001).</p><p><b>CONCLUSION</b>Flow cytometry plays a significant role in the evaluation of the quality of human sperm, and can be adopted as a useful tool in the diagnosis and treatment of male infertility.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Physiologie
/
Mobilité des spermatozoïdes
/
Spermatozoïdes
/
Malformations
/
Chromatine
/
Études cas-témoins
/
Survie cellulaire
/
Cytométrie en flux
/
Infertilité masculine
Type d'étude:
Étude observationnelle
Limites du sujet:
Adulte
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
National Journal of Andrology
Année:
2006
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS