Construction and identification of plasmid vector encoding two survivin shRNA / 中华男科学杂志
National Journal of Andrology
; (12): 512-515, 2006.
Article
de Zh
| WPRIM
| ID: wpr-343585
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct and identify the eukaryotic expression plasmids encoding two short hairpin RNA (shRNA) of survivin for the purpose of paving the way for the studies of targeted gene therapy for prostatic carcinoma (PCa).</p><p><b>METHODS</b>Two shRNA of survivin were designed and synthesized respectively, and then both were cloned into plasmids. Finally, the recombinant plasmids were confirmed by sequencing and agarose gel electrophoresis after restriction digestion.</p><p><b>RESULTS</b>The recombinant plasmids encoding two survivin shRNA were constructed and the aim sequence obtained.</p><p><b>CONCLUSION</b>Successful construction of the recombinant provides a sound basis for the research of targeted gene therapy for PCa.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Plasmides
/
Transfection
/
Clonage moléculaire
/
Petit ARN interférent
/
Interférence par ARN
/
Protéines à fluorescence verte
/
Protéines IAP
/
Vecteurs génétiques
/
Génétique
/
Protéines associées aux microtubules
Type d'étude:
Diagnostic_studies
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
National Journal of Andrology
Année:
2006
Type:
Article