Correlation of epididymal protease inhibitor Eppin and Semenogelin on human ejaculated spermatozoa / 中华男科学杂志
National Journal of Andrology
;
(12): 428-434, 2006.
Article
Dans Chinois
| WPRIM
| ID: wpr-343605
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the correlation of epididymal protease inhibitor(Eppin) and Semenogelin(Sg) on human ejaculated spermatozoa.</p><p><b>METHODS</b>The experimental approaches include (1) Immunoprecipitation of Eppin with anti-Eppin from semen; (2) Colocalization of Eppin and Sg by immunofluorescence; (3) Immunoprecipitation of rEppin and rSg;(4) Far-Western blotting of rEppin and rSg;(5) Competition of saturated 125I-rSg binding to rEppin with unlabeled Sg, and direct binding of 125I-rSg to rEppin on a blot; (6) Autoradiography of 125I-rSg with rEppin.</p><p><b>RESULTS</b>Eppin-Sg complex present on the surface of human ejaculated spermatozoa, Cys-239 is the only cystein for rEppin binding rSg. Reduction and carboxymethylation of Cys-239 blocks binding of 125I-rEppin to rSg.</p><p><b>CONCLUSION</b>Our study demonstrates that Eppin and Sg bind to each other on human ejaculated spermatozoa. A disulfide linkage occurs between Sg and Eppin, indicating the specificity of binding.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Liaison aux protéines
/
Spermatozoïdes
/
Protéines recombinantes
/
Protéines
/
Chimie
/
Protéines sécrétoires des vésicules séminales
/
Protéines sécrétoires inhibitrices de protéinases
/
Métabolisme
Limites du sujet:
Humains
/
Mâle
langue:
Chinois
Texte intégral:
National Journal of Andrology
Année:
2006
Type:
Article
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