Effect of insulin, cAMP, and dexamethasone on phosphoenolpyruvate carboxykinase gene promoter in vitro / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 639-642, 2004.
Article
Dans Chinois
| WPRIM
| ID: wpr-343792
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of insulin, cyclic adenosine monophosphate (cAMP), and dexamethasone (DEX) on 550 bp (-600 -/+ 69) fragment of phosphoenolpyruvate carboxykinase (PEPCK) gene promoter by reporter gene.</p><p><b>METHODS</b>The recombinant pGL2-PEPCK-Luc and the control plasmid pSV-beta-Galactosidase were co-transfected to rat hepatoma cell line (CBRH7919) by lipofectin. By measuring luciferase activity, we evaluated in vitro regulation of PEPCK gene promoter on reporter gene transcription.</p><p><b>RESULTS</b>cAMP and DEX stimulated PEPCK promoter obviously; meanwhile, they also had accumulative effects. At different physiological concentrations, insulin had a suppressive effect on PEPCK promoter, which was dose-independent.</p><p><b>CONCLUSION</b>There is a perfect feedback mechanism for PEPCK promoter in hepatoma cell. 550 bp (-600 -/+ 69) fragment of PEPCK may be a candidate gene in the gene therapy of diabetes.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Pharmacologie
/
Phosphoenolpyruvate carboxykinase (GTP)
/
Protéines recombinantes
/
Dexaméthasone
/
Transfection
/
Régulation de l'expression des gènes codant pour des enzymes
/
Régions promotrices (génétique)
/
AMP cyclique
/
Lignée cellulaire tumorale
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Acta Academiae Medicinae Sinicae
Année:
2004
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS