Establishment of the control substance of plant drug and fingerprints of Coptis chinensis / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 622-627, 2004.
Article
Dans Chinois
| WPRIM
| ID: wpr-343795
ABSTRACT
<p><b>OBJECTIVE</b>To establish the control substance of plant drug (CSPD) of Coptis chinensis Franch. and its proton nuclear magnetic resonance (1H NMR) and high performance liquid chromatography (HPLC) fingerprints for the purpose of original identification.</p><p><b>METHODS</b>The CSPD and their 1H NMR and HPLC fingerprints of Coptis chinensis were obtained by standardized procedure. Chemical components were isolated from the CSPD by silica gel column chromatography. By elucidation of their structures, the assignments of the characteristic signals in fingerprints could be achieved. RESULTS; The 1H NMR and HPLC fingerprints of the samples from various sources had wonderful reproducibility and characteristic features. Furthermore, five main compounds were isolated from CSPD and their structures were authenticated by spectral analysis as palmatine chloride, berberine chloride, epiberberine chloride, coptisine chloride, and jatrorrhizine chloride, respectively. The 1H NMR and HPLC fingerprints of the CSPD of Coptis chinensis showed mainly the characteristic signals of the berberine-type compounds isolated in this work.</p><p><b>CONCLUSION</b>The 1H NMR and HPLC fingerprints of the CSPD of Coptis chinensis exhibit the structures and total composition of the main active constituents in it, and can be used for its original identification and quality evaluation.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Plantes médicinales
/
Contrôle de qualité
/
Berbérine
/
Alcaloïdes de type berbérine
/
Spectroscopie par résonance magnétique
/
Chimie
/
Chromatographie en phase liquide à haute performance
/
Coptis
Type d'étude:
Étude pronostique
langue:
Chinois
Texte intégral:
Acta Academiae Medicinae Sinicae
Année:
2004
Type:
Article
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