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Study on the expansion of megakaryocyte progenitors in vitro from cord blood / 中国实验血液学杂志
Journal of Experimental Hematology ; (6): 660-663, 2005.
Article Dans Chinois | WPRIM | ID: wpr-343910
ABSTRACT
This study was aimed to investigate the effect of various cytokines on megakeryocytes expansion in vitro from human cord blood CD34(+) cells in order to establish an optimal culture system for MK expansion. Mononuclear cells were obtained by Ficoll-Hapaque density gradient separation. CD34(+) cells were positively isolated using a CD34 progenitor cell isolation kit. CD34(+) cells were placed into 24 well plates at a concentration of 2 x 10(4) per well. Each well contained 1 ml of IMDM with the present of effective MK cells growth cytokines. Clonogenic potentials of MK progenitor were assayed using a methylcellulose cultures system. The results suggested that four cytokines (IL-3 + IL-6 + TPO + FLT3L) culture system could effectively induce and expand cord blood CD41(+) MK cells. The number of CD41(+) cells expanded 154.67 +/- 32.21-fold on day 7, and 193.23 +/- 25.24-fold on day 14. In conclusion, established expansion system in vitro for MK cells provides experimental foundation for recovery of platelets after cord blood transplantation.
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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Pharmacologie / Cellules souches / Thrombopoïétine / Plaquettes / Mégacaryocytes / Différenciation cellulaire / Cellules cultivées / Interleukine-6 / Interleukine-3 / Antigènes CD34 Limites du sujet: Humains langue: Chinois Texte intégral: Journal of Experimental Hematology Année: 2005 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Pharmacologie / Cellules souches / Thrombopoïétine / Plaquettes / Mégacaryocytes / Différenciation cellulaire / Cellules cultivées / Interleukine-6 / Interleukine-3 / Antigènes CD34 Limites du sujet: Humains langue: Chinois Texte intégral: Journal of Experimental Hematology Année: 2005 Type: Article