Determination of icaritin in rat plasma by HPLC-MS/MS / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1140-1144, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-344042
ABSTRACT
The paper is to report the development of a high-performance liquid chromatographic/tandem mass spectrometry (HPLC-MS/MS) method for the determination of icaritin (ICT) in rat plasma. After precipitated with acetonitrile from the plasma, ICT was isolated chromatographically on a Dikma C18 column. The mobile phase consisted of acetonitrile-water-acetic acid (72 28 1.5, v/v/v). Electrospray ionization (ESI) source was applied and operated in the positive ion mode. Multiple reaction monitoring (MRM) mode with the transitions of m/z 387 --> m/z 313 and m/z 331 --> m/z 315 were used to quantify ICT and the internal standard, respectively. The linear calibration curve was obtained in the concentration range of 2.5-1,000 ng x mL(-1). The lower limit of quantification was 2.5 ng x mL(-1). The inter- and intra-day precision (RSD) were less than 9.63%, and the accuracy (relative error) was within +/-7.42%. The method was proved to be suitable for the pharmacokinetics of ICT, which offers advantages of high sensitivity and selectivity.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Plantes médicinales
/
Flavonoïdes
/
Sang
/
Pharmacocinétique
/
Chimie
/
Administration par voie orale
/
Reproductibilité des résultats
/
Chromatographie en phase liquide à haute performance
/
Sensibilité et spécificité
/
Rat Wistar
Type d'étude:
Etude diagnostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Acta Pharmaceutica Sinica
Année:
2009
Type:
Article
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