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Purification of enramycin by macroporous resin adsorption and reversed phase chromatography purification / 生物工程学报
Chinese Journal of Biotechnology ; (12): 1701-1708, 2014.
Article Dans Chinois | WPRIM | ID: wpr-345553
ABSTRACT
Enramycin is a polypeptide antibiotic and new, safe animal feed additive. A new purification process was developed, based on pre-purification by macroporous resin and refining by reversed phase chromatography. AB-8 macroporous resin was used for the pre-purification process of enramycin, with an elution buffer of 0.012 mol/L aqueous HCl solution-methanol (50 50, V/V). Then, enramycin a and enramycin b were separated effectively by C18 reversed phase chromatography, with a elution buffer of 0.05 mol/L aqueous KH2PO4 solution-acetonitrile (70 30, V/V, pH 4.5). The purities of enramycin a and enramycin b were up to 98.5% and 98.0%, respectively. The yield reached 29.2%. This study would provide a useful reference for the preparation of enramycin a and enramycin b with a high purity.
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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Peptides / Adsorption / Chromatographie en phase inverse / Méthodes / Antibactériens langue: Chinois Texte intégral: Chinese Journal of Biotechnology Année: 2014 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Peptides / Adsorption / Chromatographie en phase inverse / Méthodes / Antibactériens langue: Chinois Texte intégral: Chinese Journal of Biotechnology Année: 2014 Type: Article