Enzymatic production of α-ketoglutaric acid by L-glutamate oxidase from L-glutamic acid / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1318-1322, 2014.
Article
Dans Chinois
| WPRIM
| ID: wpr-345592
ABSTRACT
We produced α-ketoglutaric acid (α-KG) from L-glutamic acid, using enzymatic transformation approach with L-glutamate oxidase (LGOX). First, wild strain Streptomyces sp. FMME066 was mutated with NTG, a genetically stable mutant Streptomyces sp. FMME067 was obtained. Under the optimal nutrition conditions with fructose 10 g/L, peptone 7.5 g/L, KH2PO4 1 g/L and CaCl2 0.05 g/L, the maximum LGOX activity reached 0.14 U/mL. The LGOX was stable to pH and temperature, and Mn2+ had a stimulating effect. Finally, after 24 h enzymatic conversion under the optimal conditions, the maximum titer of α-KG reached 38.1 g/L from 47 g/L L-glutamic acid. Enzymatic transformation by LGOX is a potential approach for α-KG production.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Streptomyces
/
Acide glutamique
/
Fermentation
/
Génétique
/
Amino-acid oxidoreductases
/
Acides cétoglutariques
/
Métabolisme
langue:
Chinois
Texte intégral:
Chinese Journal of Biotechnology
Année:
2014
Type:
Article
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