Study on high-rate and low-rate metastatic large-cell lung cancer cell lines via differential display reverse transcript PCR / 中国肺癌杂志

ABSTRACT

<p><b>BACKGROUND</b>To investigate the differentially expressed gene profile between high-rate and low-rate metastatic large-cell lung cancer cell lines via differential display reverse transcript PCR (DDRT-PCR).</p><p><b>METHODS</b>DDRT-PCR was performed in two large-cell lung cancer cell lines with different metastatic ability. The differentially displayed cDNA were cloned and sequenced and compared with the sequences in the GenBank.</p><p><b>RESULTS</b>Fifty differentially displayed cDNAs were acquired, including 18 function-known genes, 16 function-unknown genes, 16 genomic DNA fragments and 4 ESTs.</p><p><b>CONCLUSIONS</b>The cell lines have different gene expression profiles, and metastasis is affected by multi-gene expression and regulation. Some genes which contribute to the metastasis of lung cancer could be found by DDRT-PCR, and this study gives some new clues to cancer diagnosis and therapy.</p>