Differential expression of proteins in rat mesenchymal stem cells undergoing endothelial differentiation / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 34-37, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-345950
ABSTRACT
<p><b>OBJECTIVE</b>To screen and identify differentially expressed proteins of mesenchymal stem cells (MSC) during endothelial differentiation.</p><p><b>METHODS</b>MSCs were induced to endothelial differentiation with vascular endothelial growth factor (VEGF) and epithelial growth factor (EGF) mixture. The whole cell proteins were extracted and isolated by two-dimensional gel electrophoresis. After gel was analyzed by Imagemaster 5.0 software, differentially expressed proteins were partially selected and identified by MALDI-TOF-MS.</p><p><b>RESULTS</b>The differentiated MSC highly expressed endothelial cells related markers, CD31, CD34 and FVIIIAg were 56.8%, 38.8% and 14.5% respectively by flow cytometer. Compared with the primary cultured MSC, the differentiated cells differentially expressed 91 proteins. Among the 19 identified proteins, 11 up-regulated and 8 down-regulated, which include cytoskeletal proteins, such as myosin, filamin, vimentin and vinculin; cell metabolism enzymes, such as ORP-150, ERO1-α, Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, protein disulfide-isomerase A3, FAS and enolase 3; nuclear factors, such as TAR DNA binding protein, guanine nucleotide binding protein and hypoxia up-regulated protein 1; VEGF receptors, such as KDR and so on.</p><p><b>CONCLUSIONS</b>Cytoskeletal proteins, metabolism enzymes and KDR were all involved in endothelial differentiation of MSC. These proteins may be the regulatory targets for endothelial differentiation of MSC.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Cellules de la moelle osseuse
/
Différenciation cellulaire
/
Cellules cultivées
/
Rat Wistar
/
Protéome
/
Biologie cellulaire
/
Protéomique
/
Cellules endothéliales
/
Cellules souches mésenchymateuses
/
Métabolisme
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Hematology
Année:
2012
Type:
Article
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