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Study of hypermethylation of SOCS gene in typical myeloproliferative disease / 中华血液学杂志
Chinese Journal of Hematology ; (12): 772-776, 2011.
Article Dans Chinois | WPRIM | ID: wpr-345993
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the clinical role of hypermethylation of suppressor of cytokine signaling (SOCS) on typical myeloproliferative disease (MPD) patients and its mechanism.</p><p><b>METHODS</b>Methylation specific PCR was used to detect SOCS1, 2, 3 methylation, direct DNA sequencing was performed to detect JAK2V617F mutation, real-time fluorescence quantitative PCR were applied to evaluate transcriptional activity of SOCS1, 2, 3.</p><p><b>RESULTS</b>Among 100 MPD patients, hypermethylation of SOCS1 was detected in 27 (27%), hypermethylation of SOCS2 in 9 (9%), hypermethylation of SOCS3 in 34 (34%); JAK2V617F mutation in 64 (64%). Hypermethylation of SOCS1, 3 greatly inhibited gene expression compared with unmethylated ones (P < 0.05). Presence of JAK2V617F mutation markedly down-regulated SOCS1, 3 gene mRNA expression compared with wild JAK2V617F (P < 0.05).</p><p><b>CONCLUSION</b>Hypermethylation of SOCS1, 3 and JAK2V617F mutation exist in MPD, which inhibited SOCS1, 3 gene expression. SOCS hypermethylation and JAK2V617F mutation can activate JAK-STAT signaling pathways, these observations may provide a potential therapeutic direction.</p>
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: ARN messager / Transduction du signal / Méthylation de l&apos;ADN / Protéines SOCS / Kinase Janus-2 / Génétique / Métabolisme / Mutation / Syndromes myéloprolifératifs Limites du sujet: Adolescent / Adulte / Adulte très âgé / Aged80 / Femelle / Humains / Mâle langue: Chinois Texte intégral: Chinese Journal of Hematology Année: 2011 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: ARN messager / Transduction du signal / Méthylation de l&apos;ADN / Protéines SOCS / Kinase Janus-2 / Génétique / Métabolisme / Mutation / Syndromes myéloprolifératifs Limites du sujet: Adolescent / Adulte / Adulte très âgé / Aged80 / Femelle / Humains / Mâle langue: Chinois Texte intégral: Chinese Journal of Hematology Année: 2011 Type: Article