Study on rapid screening and identifying hepatotoxic compounds in toosendan fructus / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1820-1822, 2013.
Article
Dans Chinois
| WPRIM
| ID: wpr-346492
ABSTRACT
Fluorescein diacetate-labeled HepG2 cells model and flouresence automatic microscopy screening assay were used for fast screening 23 components from Toosendan Fructus, in which 5 components showed significant toxicity on HepG2 cells. The 10 compounds in the 2 components were tentatively identified with LC-MS(n), and 3 of them (meliasenin B, trichilinin D and 1-O-tigloy-1-O-debenzoylohchinal) were prepared and identified. Further experiments showed that the 3 compounds displayed dose-dependent toxicity on HepG2 cells, suggesting that these compounds in Toosendan Fructus may cause hepatotoxicity.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Médicaments issus de plantes chinoises
/
Chimie
/
Chromatographie en phase liquide à haute performance
/
Melia
/
Effets secondaires indésirables des médicaments
/
Toxicité
/
Cellules HepG2
/
Fruit
/
Foie
Type d'étude:
Etude diagnostique
/
Étude de dépistage
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2013
Type:
Article
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