The anti-proliferation and anti-migration dual effects of aloe-emodin on KB cells and its mechanism / 中华口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the anti-proliferation and anti-migration dual effects of aloe-emodin on KB cells and its mechanisms.</p><p><b>METHODS</b>KB cells were treated with 2.5, 5, 10, 20 and 40 micromol/L aloe-emodin. Crystal violet assay was used to determine the long-term growth inhibition of aloe-emodin on human oral cancer KB cells. Scratch wound-healing motility assay was used to measure the antimigration effect The protein kinase C alpha and c-myc expression changes in protein levels were detected by Western blotting.</p><p><b>RESULTS</b>A durable cell growth inhibitory effect of aloe-emodin on KB cells was found. Treatment of aloe-emodin resulted in the inhibition of cell migration. The protein kinase C alpha and c-myc in protein levels were decreased upon treatment with aloe-emodin compared with controls.</p><p><b>CONCLUSIONS</b>The anti-proliferation and anti-migration effects of aloe-emodin on KB cells are associated with the suppression of protein kinase C pathway.</p>