Effect on different concentrations of exogenous hormones on baicalin in Scutellaria baicalensis callus / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To determine the content of baicalin in Scutellaria baicalensis callus induced by different doncentrations of exogenous hormones.</p><p><b>METHOD</b>HPLC system was adopted to determine baicalin in S. baicalensis callus. Chromatographic conditions ODS column was adopted, with methanol-water-phosphate (47 53 0.2) as the mobile phase. The flow velocity was 1 mL x min(-1), the detective wavelength was 280 nm, and the temperature of column was room temperature.</p><p><b>RESULT</b>S. baicalensis callus induced by 6-BA 1.0 mg x L(-1) + NAA 0.5 mg x L(-1) showed the highest baicalin content, up to 49.78 mg x g(-1).</p><p><b>CONCLUSION</b>The experiment is such a simple, rapid and stable method for determining the baicalin content that it can be used for determining the baicalin content in S. baicalensis callus.</p>