Effect of Zuoguiyin on expression of ovarian VEGF and SPARC in rats during peri-menopausal period / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2932-2936, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-346994
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of one kind of regulating-qi and Nourishing-yin Chinese herbs Zuoguiyin on the expression of rats ovarian vascular endothelial growth factor (VEGF) and secreted protein acidic rich in cysteine (SPARC) during the period of peri-menopause.</p><p><b>METHOD</b>The animal models of perimenopause rats were established by natural aging. Perimenopause rats were treated by intragastric administration (ig) with low (13.78 g x kg(-1)), middle (20.67 g x kg(-1)) and high (31 g x kg(-1)) dose of Zuoguiyin for 8 weeks. Expression of VEGF and SPARC mRNA were detected by RT-PCR. Immunohistochemistry was used to evaluate expression levels of VEGF protein.</p><p><b>RESULT</b>Compared with that in the control group, ovarian expression levels of VEGF mRNA and its protein in rats during peri-menopause increased significantly (P < 0.01). Middle and high dose of Zuoguiyin could both down-regulate the expression of VEGF in ovaries of praesenilis rats, and the difference has statistical significance (P < 0.01). Expression levels of SPARC mRNA in rat ovaries during peri-menopausal period decreased obviously compared to that in the control group (P < 0.01). Middle and high dose of Zuoguiyin could greatly promote ovarian SPARC mRNA expression of praesenilis rats (P < 0.01).</p><p><b>CONCLUSION</b>The abnormal changes of VEGF and SPARC may play an important role in the aging process of ovary. Zuoguiyin, one kind of regulating-qi and Nourishing-yin Chinese herbs, can improve ovarian vascular formation through down-regulating the expression of VEGF meanwhile up-regulating the expression of SPARC. And it may delay aging by this way.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Ovaire
/
Pharmacologie
/
Médicaments issus de plantes chinoises
/
Expression des gènes
/
Ostéonectine
/
Rat Wistar
/
Modèles animaux
/
Facteur de croissance endothéliale vasculaire de type A
/
Périménopause
/
Génétique
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
/
Femelle
/
Humains
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2009
Type:
Article
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