Effects of different concentrations of gubishu containing serum on the proliferation of rabbit articular chondrocytes in vitro culture / 中国骨伤
China Journal of Orthopaedics and Traumatology
;
(12): 841-844, 2011.
Article
Dans Chinois
| WPRIM
| ID: wpr-347052
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of different concentrations of Gubishu containing serum on the proliferation of rabbit articular chondrocytes cultured in vitro.</p><p><b>METHODS</b>Articular chondrocytes were obtained from the cartilage of 1-month rabbit and cultured in vitro. They were randomly divided into 8 groups,blank and Gubishu groups in different concentrations (5%, 10%,15%, 20%), MTT assay method was adopted to observe the influence of Gubishu containing serum with different concentrations to the proliferation of chondrocytes after incubated 1, 3, 5, 7 and 9 days.</p><p><b>RESULTS</b>The proliferation of chondrocytes was dependent on the concentration in Gubishu groups. At same time point,there was significant value between every groups, 20% concentration was greatest (P<0.05); There was significant differences between 5%, 10% and 20% concentration of the blank groups at same time point (P<0.05), and was not between 15% and 20% concentration at the 1, 3, 5 and 7 days (P>0.05), 20% concentration of the blank group was greatest. 20% concentrations of Gubishu containing serum was significantly greater than 20% concentrations of blank group at the 1, 3, 5 and 7 days (P<0.05).</p><p><b>CONCLUSION</b>20% concentrations of Gubishu containing serum can significantly increase the proliferation of chondrocytes, and bring the logarithmic growth period forward to the 3 day.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Physiologie
/
Médicaments issus de plantes chinoises
/
Cartilage articulaire
/
Cellules cultivées
/
Chondrocytes
/
Biologie cellulaire
/
Sérum
/
Prolifération cellulaire
/
Relation dose-effet des médicaments
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
China Journal of Orthopaedics and Traumatology
Année:
2011
Type:
Article
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