Effect of p16 gene on carcinogenesis of hepatitis B virus related hepatocellular carcinoma / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 356-358, 2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-347426
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the relation between p16 gene expression and the carcinogenesis and progress of hepatitis B virus (HBV) related hepatocellular carcinoma (HCC).</p><p><b>METHODS</b>In 35 specimens of HCC tissue and the adjacent liver tissue, the integration of HBV X gene was detected by polymerase chain reaction (PCR) and Southern blot. The point mutation of exon-1alpha, 2 and 3 of p16 gene were detected by PCR-single strand conformation polymorphism (SSCP). The expression of p16 mRNA and p16 protein was detected by RT-PCR and Western blot.</p><p><b>RESULTS</b>The integration of X gene correlated with the expression loss of p16 mRNA and p16 protein in HCC (P < 0.05). The expression loss rates of p16 protein in HCC and adjacent tissues were 62.9% (22/35) and 40.0% (14/35) with significant difference (P < 0.05). The expression loss of p16 protein in HCC correlated with the differentiation degrees of HCC and the infiltration of tumor cells (P < 0.05).</p><p><b>CONCLUSION</b>The integration of X gene correlates with the expression loss of p16 protein. The alteration of p16 gene, playing an important role in all stages of hepatocarcinogenesis, correlates with the progress and invasion of hepatocellular carcinoma.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Virologie
/
ARN messager
/
Transactivateurs
/
Virus de l'hépatite B
/
Exons
/
Intégration virale
/
Mutation ponctuelle
/
Carcinome hépatocellulaire
/
Gènes p16
/
Inhibiteur p16 de kinase cycline-dépendante
Limites du sujet:
Femelle
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Oncology
Année:
2003
Type:
Article
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