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Detection of chromosomal aberration in paraffin-embedded synovial sarcoma tissues by fluorescence in-situ hybridization / 中华病理学杂志
Chinese Journal of Pathology ; (12): 577-581, 2007.
Article Dans Chinois | WPRIM | ID: wpr-347725
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the application of fluorescence in-situ hybridization (FISH) in detection of gene translocation in paraffin-embedded tissue samples of synovial sarcoma.</p><p><b>METHODS</b>Interphase FISH was carried out in paraffin-embedded tissue of 42 cases of synovial sarcoma and 9 cases of non-synovial sarcoma, using a LSI SYT (18q11.2) dual color break-apart probe. In all of the cases studied, the gene fusion product SYT-SSX was also analyzed by reverse transcription-polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>Positive signals were detected in 37 cases (88.1%) of synovial sarcoma by FISH, as compared with 35 cases (83.8%) by RT-PCR and 39 cases (92.9%) by both techniques. Of the 39 positive cases, 33 cases (78.5%) revealed SYT gene translocation.</p><p><b>CONCLUSIONS</b>FISH may serve as an adjunctive diagnostic tool in problematic cases of synovial sarcoma and can be applied in paraffin-embedded tissue samples. As compared with RT-PCR, FISH is also sensitive and reliable. The methodology is less labor intensive and time consuming. FISH has great potential in molecular diagnosis of soft tissue tumors.</p>
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Anatomopathologie / Tumeurs des tissus mous / Marqueurs biologiques tumoraux / Protéines de fusion oncogènes / Aberrations des chromosomes / Inclusion en paraffine / Hybridation fluorescente in situ / Sarcome synovial / RT-PCR / Membre inférieur Type d'étude: Etude diagnostique Limites du sujet: Adolescent / Adulte / Enfant / Enfant d'âge préscolaire / Femelle / Humains / Mâle langue: Chinois Texte intégral: Chinese Journal of Pathology Année: 2007 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Anatomopathologie / Tumeurs des tissus mous / Marqueurs biologiques tumoraux / Protéines de fusion oncogènes / Aberrations des chromosomes / Inclusion en paraffine / Hybridation fluorescente in situ / Sarcome synovial / RT-PCR / Membre inférieur Type d'étude: Etude diagnostique Limites du sujet: Adolescent / Adulte / Enfant / Enfant d'âge préscolaire / Femelle / Humains / Mâle langue: Chinois Texte intégral: Chinese Journal of Pathology Année: 2007 Type: Article