MAPK signal pathway plays a role in proliferation of Jurkat cells induced by ouabain / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 126-129, 2005.
Article
Dans Chinois
| WPRIM
| ID: wpr-347812
ABSTRACT
The object was to study the effect of ouabain on Jurkat cells and its possible mechanism. The effect of ouabain of low concentration on Jurkat cells was confirmed by MTT, while c-myc gene transcription was measured by RT-PCR, and the phosphorylation of MAPK (ERK1/2) as well as the expression of c-myc gene was tested by Western blot respectively. The results showed that ouabain at low concentration could induce the proliferation of Jurkat in a time-and dose-dependent manner. At the same time, the phosphorylation of MAPK (ERK1/2) and the expression of c-myc gene was enhanced. In conclusion, ouabain stimulates the intracellular MAPK signal pathway by acting on the Na, K-ATPase, and thus induce the proliferation of Jurkat cells, in which the regulation of c-myc gene expression may be involved.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Ouabaïne
/
Pharmacologie
/
Phosphorylation
/
Physiologie
/
Facteurs temps
/
ARN messager
/
Technique de Western
/
Protéines proto-oncogènes c-myc
/
Cellules Jurkat
/
RT-PCR
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Experimental Hematology
Année:
2005
Type:
Article
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