Identification bcr-abl fusion gene in leukemia cells with oligonucleotide microarray / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 743-747, 2004.
Article
Dans Chinois
| WPRIM
| ID: wpr-347871
ABSTRACT
To explore the application value of bcr-abl fusion gene deterction microarray in diagnosis, typing, choosing of treatment variant and prognosis judgment, probe for fusion gene detection was designed, oligonucleotide microarray was prepared; total RNA was extracted, reverse-transcripted and labeled by fluorescence, then cDNA was hybridized with microarray in order to detect bcr-abl fusion gene in leukemia cells. The results showed that better reaction conditions were gained by exploration of hybridizotion temperature and elution conditions, bcr-abl fusion gene in leukemia cells was detected by prepared miccroarray. In conclusion, oligonucleotide microarray is effective in detecting the fusion gene and has some unique advantages and certain clinical application value, but has some deficiency too. If microarray can be improved further, it could be used widely in the field of hematology.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Leucémies
/
Régulation de l'expression des gènes dans la leucémie
/
Reproductibilité des résultats
/
Protéines de fusion bcr-abl
/
Cellules HL-60
/
Cellules K562
/
Séquençage par oligonucléotides en batterie
/
Génétique
/
Méthodes
Type d'étude:
Etude diagnostique
/
Étude pronostique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Journal of Experimental Hematology
Année:
2004
Type:
Article
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