Expression of WAVE1 and p22phox in children with acute lymphocytic leukemia and the relationship of WAVE1 with oxidative stress / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 88-92, 2009.
Article
Dans Chinois
| WPRIM
| ID: wpr-347995
ABSTRACT
<p><b>OBJECTIVE</b>To study the expression of WAVE1 and p22phox in peripheral blood mononuclear cells (PBMCs) in children with acute lymphocytic leukemia (ALL) and the relationship of WAVE1 with oxidative stress.</p><p><b>METHODS</b>Real-time PCR was used for detecting WAVE1 and p22phox expression in PBMCs in 41 children with ALL and 10 normal controls. Plasma activity of superoxide dismutase (SOD) was measured by the xanthine oxidase method. Plasma activity of GSH-Px was measured by the DTNB reaction test.</p><p><b>RESULTS</b>The expression of WAVE1 and p22phox was significantly higher in the active ALL groups (newly diagnosed and relapse ALL) than that in the normal control and the complete remission (CR) ALL groups (<0.01). The CR ALL group showed increased WAVE1 and p22phox expression than those in the normal control group (<0.05). Plasma activities of SOD (22.62+/-7.39 U/mL) and GSH-Px (91.73+/-28.88 micromol/L) in the active ALL group were significantly lower than those in the normal control (166.35+/-27.93 U/mL and 490.94+/-39.38 micromol/L, respectively) and the CR ALL groups (107.11+/-28.57 U/mL and 267.56+/-82.64 micromol/L, respectively) (<0.01). WAVE1 expression was positively correlated with p22phox expression (r=0.34, <0.05) but negatively correlated with plasma activities of SOD and GSH-Px ( r=-0.336 and-0.408, respectively; <0.05).</p><p><b>CONCLUSIONS</b>WAVE1 and p22phox expression in PBMCs increased and was associated with the disease course in children with ALL. Oxidative stress may be involved in the regulation of WAVE1 expression in ALL children.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Superoxide dismutase
/
Sang
/
ARN messager
/
Agranulocytes
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Réaction de polymérisation en chaîne
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Stress oxydatif
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NADPH oxidase
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Famille de protéines du syndrome de Wiskott-Aldrich
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Leucémie-lymphome lymphoblastique à précurseurs B et T
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Génétique
Limites du sujet:
Adolescent
/
Enfant
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Enfant d'âge préscolaire
/
Femelle
/
Humains
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Bébé
/
Mâle
langue:
Chinois
Texte intégral:
Chinese Journal of Contemporary Pediatrics
Année:
2009
Type:
Article
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