Ectopic bone induction in vivo after transplantation of skeletal satellite cells from green fluorescence protein transgenic mouse transfected by adenoviral vectors encoding bone morphogenetic protein-2 / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 504-507, 2007.
Article
Dans Chinois
| WPRIM
| ID: wpr-348007
ABSTRACT
<p><b>OBJECTIVE</b>To observe the ability of induced ectopic bone using skeletal muscles satellite cells (SMSCs) from newborn green fluorescence protein (GFP) transgenic mice mediated by Ad-BMP2.</p><p><b>METHODS</b>Transplantation of SMSCs transduced with Ad-BMP2 into back lamb muscles of subfascia in wildtype 129sv mice with a complex of collagen scaffords, then the tissue histologic examination, X ray plain film, fluorescence microscopy were used.</p><p><b>RESULTS</b>Transplantation of SMSCs transfected with Ad-BMP2 into back lamb muscles of subfascia generated ectopic bone formation involving GFP-positive osteoblasts and osteocytes 2 weeks and mature bone formation 4 weeks after transplantation. SMSCs non-transfected with Ad-BMP2 failed to induce ectopic bone formation.</p><p><b>CONCLUSION</b>SMSCs retain differentiation potentitality into osteoblasts in response to Ad-BMP2. They are useful tools for analyzing the process of osteoblast differentiation in vivo after transplantation.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Ostéoblastes
/
Os et tissu osseux
/
Souris transgéniques
/
Transfection
/
Différenciation cellulaire
/
Facteur de croissance transformant bêta
/
Protéines morphogénétiques osseuses
/
Myoblastes
/
Protéines à fluorescence verte
/
Protéine morphogénétique osseuse de type 2
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
West China Journal of Stomatology
Année:
2007
Type:
Article
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