Induction of drug resistance in Tca8113 cell line by exposing to chemotherapy drug / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the drug resistance changes in Tca8113 cell lines by exposing to carboplatin.</p><p><b>METHODS</b>The concentration of carboplatin added to Tca8113 cells was increased gradually and continually, which was to induce the carhoplatin-resistance in Tca8113 cells. The sensibility to drugs of the cells was analyzed by MTT method. Immunocytochemistry and RT-PCR were utilized to examine the expression of multidrug resistance proteins and genes.</p><p><b>RESULTS</b>After exposing to carboplatin, the Tca8113/CBP cells had higher drug-resistance to CBP, MTX, PYM, VCR and higher expression of MRP, GST-pi than Tca8113 cells.</p><p><b>CONCLUSION</b>Multidrug resistance of Tca8113/CBP is associated with over expression of MRP, GST-pi and MDR. Tca8113/CBP can provide an ideal model for multidrug resistance research.</p>