Study of the down-regulating effect of hepatitis C virus NS5A protein on NACA promoter / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 579-581, 2005.
Article
Dans Chinois
| WPRIM
| ID: wpr-348725
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of hepatitis C virus (HCV) non-structure protein NS5A on the activity of calcium-regulating protein alpha subunit of nascent polypeptide-associated complex (NACA) promoter.</p><p><b>METHODS</b>HepG2 cell plasmid pCAT3-NACA, containing NACA promoter, was transfected alone or cotransfected with pcDNA3.1(-)-NS5A, and chloramphenicol acetyl transferase (CAT) enzyme activity was assayed by enzyme-linked immunoassay (ELISA).</p><p><b>RESULTS</b>The CAT activity in the pcDNA3.1(-)-NS5A cotransfection group was 20.7% of the CAT activity in the pCAT3-NACA group.</p><p><b>CONCLUSION</b>HCV non-structural protein NS5A has a down-regulating effect on the promoter of NACA gene.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Pharmacologie
/
Données de séquences moléculaires
/
Séquence nucléotidique
/
Transfection
/
Régulation négative
/
Activation de la transcription
/
Régions promotrices (génétique)
/
Protéines virales non structurales
/
Chaperons moléculaires
/
Génétique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Hepatology
Année:
2005
Type:
Article
Documents relatifs à ce sujet
MEDLINE
...
LILACS
LIS