Determination of protein binding rate of oleanolic acid in human plasma and serum albumin / 药学学报
Acta Pharmaceutica Sinica
;
(12): 243-246, 2011.
Article
Dans Chinois
| WPRIM
| ID: wpr-348970
ABSTRACT
A LC-MS method was established for the determination of the protein binding rates of oleanolic acid in human plasma and serum albumin. The equilibrium dialysis combined with LC-MS to determine the total concentration in plasma and free drug concentration of oleanolic acid was carried out. The human plasma protein binding rates of oleanolic acid at three concentrations were 79.6%, 81.9% and 63.3%, respectively. The human serum albumin protein binding rates of oleanolic acid at three concentrations were 53.5%, 56.6% and 47.7%, respectively. The method is shown to be simple, accurate, sensitive and specific for the determination of biological samples. The protein binding rates in human plasma and serum albumin were of high strength.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Acide oléanolique
/
Liaison aux protéines
/
Spectrométrie de masse
/
Sang
/
Sérumalbumine
/
Chromatographie en phase liquide
/
Sensibilité et spécificité
/
Dialyse
/
Métabolisme
/
Méthodes
Type d'étude:
Etude diagnostique
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Acta Pharmaceutica Sinica
Année:
2011
Type:
Article
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