Study on the correlation of GCS and MDR1 genes in inducing multidrug resistance in human K562/A02 cell line / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
;
(6): 299-304, 2010.
Article
Dans Chinois
| WPRIM
| ID: wpr-348992
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the correlation of glucosylceramide synthase (GCS) gene and multidrug resistance 1 (MDR1) gene in inducing multidrug resistance in human multidrug-resistant K562/A02 cell line, and search for a novel strategy for reversing multidrug resistance of leukemia cells.</p><p><b>METHODS</b>The expression levels of GCS and MDR1 mRNA in K562 and K562/A02 cells were assayed by RT-PCR. siRNAs targeting the GCS and MDR1 gene were transfected into K562/A02 cells with liposome, respectively. The differential expression of GCS and MDR1 mRNAs, as well as their correlation, were detected by RT-PCR and real time quantitative-PCR(QPCR).</p><p><b>RESULTS</b>The expression level of GCS and MDR1 mRNA was dramatically lower in drug-sensitive K562 cells compared with the K562/A02 cells. The GCS mRNA was inhibited by 73%(59%-82%) and MDR1 mRNA expression was down regulated by 67% (38%-82%) in K562/A02 cells after being transfected with GCS siRNA. The expression level of MDR1 mRNA was inhibited by 81%(63%-91%) and GCS mRNA expression had no apparent change in K562/A02 cells treated with MDR1 small interference RNA(siRNA).</p><p><b>CONCLUSION</b>Positive correlation was detected between the expression of GCS and MDR1 mRNA in K562/A02 cells and MDR1 mRNA expression was down regulated after silencing the GCS gene expression.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Physiologie
/
ARN messager
/
Glycoprotéine P
/
Multirésistance aux médicaments
/
Résistance aux médicaments antinéoplasiques
/
RT-PCR
/
Petit ARN interférent
/
Lignée cellulaire tumorale
/
Génétique
/
Glucosyltransferases
Limites du sujet:
Humains
langue:
Chinois
Texte intégral:
Chinese Journal of Medical Genetics
Année:
2010
Type:
Article
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