Cloning of transmembrane domain sequence of EGFR gene / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 235-238, 2002.
Article
Dans Chinois
| WPRIM
| ID: wpr-349385
ABSTRACT
OBJECTIVE:
To clone the transmembrane (TM) domain sequence of EGFR gene and lay a good foundation for constructing the transmembrane expression vector of recombinant superantigens and cytokines.METHODS:
A pair of primers special to the sequence encoding TM domain of EGFR gene were synthesized, TM domain fragment was cloned by RT-PCR, and the PCR product of TM domain sequence was ligated with the pGEM-T vector and confirmed by DNA sequencing.RESULTS:
TM domain sequence was successfully cloned and verified by DNA sequencing.CONCLUSION:
The successful cloning of TM domain sequence provides a basis for the construction of transmembrane fusion protein of Superantigen-TM or Cytokines-TM in cancer biotherapy.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Journal of Zhejiang University. Medical sciences
Année:
2002
Type:
Article
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