Cloning of human vascular endothelial growth factor cDNA and its expression in COS-7 cells / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 316-320, 2002.
Article
Dans Chinois
| WPRIM
| ID: wpr-349409
ABSTRACT
OBJECTIVE:
To clone vascular endothelial growth factor (VEGF) cDNA gene, construct its eukaryotic expression vector and to express this recombinant plasmid in COS-7 cells.METHODS:
Human VEGF165 cDNA was amplified by RT PCR from human ovarian carcinoma. After DNA sequenced, the VEGF165 cDNA was inserted into eukaryotic expression vector pcDNA3.1(-). The recombinant plasmid pcDNA3.1 VEGF165 containing VEGF165 cDNA was identified by enzyme digestion and transferred into COS-7 cells mediated by liposome. The transient expression of VEGF was detected by immunohistochemical staining.RESULTS:
The cloned VEGF165 cDNA was confirmed by enzyme digestion and DNA sequence analysis. The immunohistochemical results showed that the VEGF165 protein was expression in COS-7 cells 72 h after gene transfer.CONCLUSION:
VEGF165 cDNA gene successfully cloned and expressed in COS-7 cells.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Type d'étude:
Étude pronostique
langue:
Chinois
Texte intégral:
Journal of Zhejiang University. Medical sciences
Année:
2002
Type:
Article
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