The effect of basic fibroblast growth factor on the mRNA expression of beta1 integrin subunit by periodontal ligament fibroblasts in culture / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of basic fibroblast growth factor on the mRNA expression of beta1 integrin subunit by periodontal ligament fibroblasts in culture; to discuss the effect of basic fibroblast growth factor in periodontal regeneration.</p><p><b>METHODS</b>Human periodontal ligament fibroblasts were cultured and stimulated by basic fibroblast growth factor (0.1, 1.0, 10.0 ng x mL(-1)) for 24, 48, 72 h respectively, and then mRNA expression of beta1 integrin subunit was assessed by fluorescent quantitative real-time reverse transcription polymerase chain reaction.</p><p><b>RESULTS</b>Basic fibroblast growth factor enhanced the mRNA expression of beta1 integrin subunit, and there was optimal effect when the concentration of basic fibroblast growth factor was 1.0 ng x mL(-1) at 24, 48, 72 h respectively; the mRNA expression of beta1 integrin subunit at 72 h was higher than that at 24, 48 h.</p><p><b>CONCLUSION</b>Basic fibroblast growth factor can strengthen human periodontal ligament fibroblasts' adhesion and may be one of important factors which participate in the periodontal regeneration.</p>