Effects of high mobility group box 1 in activating periodontal ligament fibroblasts to express cytokine / 华西口腔医学杂志
West China Journal of Stomatology
; (6): 443-446, 2010.
Article
de Zh
| WPRIM
| ID: wpr-350316
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the influence of high mobility group box 1 (HMGB1) on the expression of interleukin 6 (IL-6), receptor activator of nuclear factor-kappa B ligand (RANKL) and osteoprotegerin (OPG) on periodontal ligament fibroblasts.</p><p><b>METHODS</b>Human periodontal ligament fibroblasts were stimulated with HMGB1 at concentrations of 10, 30, and 100 ng x mL(-1) for 24 h. RT-PCR and Western blot analysis were performed to check mRNA and protein expression of IL-6, RANKL and OPG on the cells.</p><p><b>RESULTS</b>The ratio of RANKL/OPG was increased at both mRNA and protein level after HMGB1 stimulation at 10, 30, 100 ng x mL(-1). Inflammatory cytokine IL-6 was upregulated by HMGB1 at the concentration of 100 ng x mL(-1).</p><p><b>CONCLUSION</b>Increased ratio of RANKL/OPG and IL-6 on periodontal ligament fibroblasts suggests that HMGB1 might play a role in the pathogenesis and progression of periodontal disease.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Desmodonte
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Cellules cultivées
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Interleukine-6
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Biologie cellulaire
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Protéine HMGB1
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Ligand de RANK
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Ostéoprotégérine
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Fibroblastes
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Métabolisme
Limites du sujet:
Humans
langue:
Zh
Texte intégral:
West China Journal of Stomatology
Année:
2010
Type:
Article