Determination of theacrine in rat plasma by RP-HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 753-756, 2013.
Article
Dans Chinois
| WPRIM
| ID: wpr-350692
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for the determination of theacrine in rat plasma after ig. administration of theacrine.</p><p><b>METHOD</b>Blood sample was taken timely from the eyes canthus of rats. Plasma was isolated and the protein was precipitated by ethyl acetate. Then the plasma concentration of theacrine was determined with RP-HPLC. Caffeine was used as the internal standard. The chromatographic conditions were as follows Phenomenex Luna C18 (4.6 mm x 250 mm, 5 microm) at 25 degrees C, a mixture of methanol-water (25 75) as the mobile phase, at the flow rate of 1.0 mL x min(-1) and the detection wavelength of 290 nm.</p><p><b>RESULT</b>The linear range of theacrine was 0.5-100 mg x L(-1) (R2 = 0.998 9). The lower limit of quantification was 0.5 mg x L(-1). The intra-day RSD was 1.49% 4.40% and inter-day RSD was 0.80% -10.27%. The average extraction recoveries of theacrine were 90.3% -95.8% at concentrations of 0.5, 5.0, 50 mg x L(-1). The main pharmacokinetic parameters after ig. administration of theacrine at concentration of 30 mg x kg(-1) were as follow C(max) (35.45 +/- 30 2.68) mg x L(-1), t(max) (0.51 +/- 0.13) h, t1/2 (3.13 +/- 1.37) h, AUC(0-infinity) (2.65.39 +/- 94.71) mg x L(-1) x h.</p><p><b>CONCLUSION</b>The method has been confirmed to be simple, stable, reproducible and with high specificity, and can be used for the pharmacokinetic study of theacrine in rats.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Acide urique
/
Sang
/
Analyse chimique du sang
/
Calibrage
/
Pharmacocinétique
/
Reproductibilité des résultats
/
Chromatographie en phase liquide à haute performance
/
Rat Sprague-Dawley
/
Chromatographie en phase inverse
/
Méthodes
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2013
Type:
Article
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