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Effect of ursolic acid on caspase-3 and PARP expression of human MCF-7 cells / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 141-144, 2006.
Article Dans Chinois | WPRIM | ID: wpr-350987
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of ursolic acid (UA), apentacyclic triterpene acid, on MCF-7 cell apoptosis, and probable mechanism involved by detecting the expressions of caspase-3 and poly ADP-ribose polymerase(PARP) at protein level.</p><p><b>METHOD</b>MCF-7 cells were cultured with different concentrations of UA. Growth inhibition of UA on MCF-7 cells was evaluated by MTT assay. Cell cycle and sub-G1 peak were performed by FCM. Morphologic changes of UA-treated cells were observed by light microscope. Apoptotic cells with condensed or fragmented nuclei were visualized by Ho 33258 staining by a fluorescence microscope (EX U. V.). The protein expression of caspase-3 and PARP was analyzed by immunofluorescence cell staining (SABC-Cy3).</p><p><b>RESULT</b>24 hours after UA treatment, inhibition of MCF-7 cell growth was concentration-dependent. The IC50 value for UA was (22.6 +/- 3.0) micromo x L(-1). Cell cycle anaysis by FCM showed that 50 micromol x L(-1) of UA arrested MCF-7 cell cycle at G0 - G1 phase. Morphological changes of MCF-7 Cells exhibited many of the hallmark features of apoptosis, including chromatin clumps and aggregation and DNA fragmentation. UA increased caspase-3 protein expression.</p><p><b>CONCLUSION</b>The results suggest that UA evokes MCF-7 cell apoptosis is correlation with the up-regulation of caspase-3. Our study indicated that UA might be a potential Chinese medical component for breast neoplasm.</p>
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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Anatomopathologie / Pharmacologie / Triterpènes / Tumeurs du sein / Cycle cellulaire / Poly(ADP-ribose) polymerases / Apoptose / Caspases / Lignée cellulaire tumorale / Caspase-3 Limites du sujet: Femelle / Humains langue: Chinois Texte intégral: China Journal of Chinese Materia Medica Année: 2006 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Anatomopathologie / Pharmacologie / Triterpènes / Tumeurs du sein / Cycle cellulaire / Poly(ADP-ribose) polymerases / Apoptose / Caspases / Lignée cellulaire tumorale / Caspase-3 Limites du sujet: Femelle / Humains langue: Chinois Texte intégral: China Journal of Chinese Materia Medica Année: 2006 Type: Article