Development and application of the physical hypoxic models of C. elegans / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 257-262, 2011.
Article
Dans Chinois
| WPRIM
| ID: wpr-351183
ABSTRACT
<p><b>OBJECTIVE</b>To develop a suitable hypoxic injury model, which is important for revealing pathological molecular mechanism of hypoxia.</p><p><b>METHODS</b>We focused on C. elegans by treatment with different hypoxic times and systematically observed mortality, movement, Cellular morphology and the related-protein expression of the animals.</p><p><b>RESULTS</b>We demonstrated that hypoxia (0.2% partial pressure of oxygen) induced morphological cell defects, and then leading to death of C. elegans. The mortality of C. elegans increased along with hypoxic time, while hypoxia-inducible factor (HIF-1) was significantly up-regulated. In addition, by using neuron-specific transgenic wonns with green fluorescent protein--we observed the neuron-specffic injury caused by hypoxic stress.</p><p><b>CONCLUSION</b>We successfully established an effective, convenient physical hypoxic model of C. elegans, which will facilitate the studies of hypoxic pathology and molecular mechanisms of hypoxic response in the future.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Physiologie
/
Facteurs de transcription
/
Caenorhabditis elegans
/
Protéines de Caenorhabditis elegans
/
Modèles animaux de maladie humaine
/
Facteur-1 induit par l'hypoxie
/
Hypoxie
/
Métabolisme
/
Neurones
Type d'étude:
Étude pronostique
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Chinese Journal of Applied Physiology
Année:
2011
Type:
Article
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