Effect of Sailuotong capsule on mitochondrial dynamics in focal cerebral ischemia/reperfusion rats / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1984-1988, 2015.
Article
Dans Chinois
| WPRIM
| ID: wpr-351227
ABSTRACT
To observe the protective effect and mechanism of Sailuotong capsule in focal cerebral ischemia/reperfusion. The 90 min middle cerebral artery occlusion (MCAO) reperfusion model was established. The expressions of dynamin-related protein 1 ( Drp1) and optic atrophy 1 (Opa1) were tested by Western blot. The transmission electron microscope was used to observe the changes in the mitochondrial ultra-structure. The pathological morphological changes were observed through the HE staining. The immunohistochemical method was used to test Drp1 and Opa1 expressions. Sailuotong capsule (33, 16.5 mg x kg(-1), ig) can inhibit the abnormal mitochondrial fission and fusion in the cortical area on the ischemia side and the mitochondrial fission gene expression and promote the mitochondrial fusion gene Opa1 expression, so as to alleviate the energy metabolism disorder caused by ischemia/reperfusion. Sailuotong capsule can inhibit the abnormal mitochondrial dynamics in peri-ischemic regions and maintain the normal morphology of mitochondria, which may be the mechanism of Sailuotong capsule in promoting the self-recovery function in the ischemic brain region.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Chirurgie générale
/
Encéphale
/
Médicaments issus de plantes chinoises
/
Encéphalopathie ischémique
/
Dynamines
/
Traitement médicamenteux
/
Génétique
/
DGTPases
/
Métabolisme
/
Mitochondries
Limites du sujet:
Animaux
/
Humains
/
Mâle
langue:
Chinois
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2015
Type:
Article
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