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Experimental study on intervention effect of Grifola frondosa on nonalcoholic steatohepatitis / 中国中药杂志
China Journal of Chinese Materia Medica ; (24): 1808-1811, 2015.
Article Dans Chinois | WPRIM | ID: wpr-351260
ABSTRACT
To study the preventive effect of Grifola frondosa on nonalcoholic steatohepatitis (NASH). The rat model of NASH was established by feeding high-fat diets for 12 weeks and intervened with 0.5 g · kg(-1) · d(-1) and 1.0 g · kg(-1) · d(-1) of C. frondosa powder suspensions. The degrees of hepatocyte fatty degeneration and inflammation were observed under the optical microscope with routine HE staining. The NAFLD activity scores (NAS) were calculated. Serum ALT, AST and hepatic TG and CHOL were tested by the biochemical method. The hepatic MDA was examined by thiobarbituric acid method. The hepatic SOD was tested by the xanthine oxidase test. The hepatic GSH-PX activity was determined by the dithio-nitrobenzoic acid method. Hepatic TNF-α and IL-6 were detected by the enzyme-linked immunosorbent assay (ELISA). The NASH model group induced by high-fat diets showed higher hepatic NAS, ser- um ALT, AST, CHOL and hepatic TG, CHOL, MDA, TNF-α, IL-6 (P < 0.01 or P < 0.05) and lower serum TG and hepatic SOD, GSH-PX (P < 0.01, P < 0.05) than the normal control group. After being intervened with different doses of G. frondosa, the NASH group revealed significantly lower hepatic NAS, serum ALT and hepatic TG, CHOL, MDA, TNF-α and IL-6 (P < 0.05) and higher hepatic SOD, GSH-PX (P < 0.05) than the model group. G. frondosa may prevent the further development of NASH by improving the disorder of lipid metabolism in rats with NASH induced by high-fat diets, relieving the level of oxidative stress and reducing the generation of inflammatory cytokines.
Sujets)
Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Médicaments issus de plantes chinoises / Chimie / Interleukine-6 / Facteur de nécrose tumorale alpha / Rat Sprague-Dawley / Stress oxydatif / Grifola / Traitement médicamenteux / Stéatose hépatique non alcoolique / Foie Type d'étude: Étude pronostique Limites du sujet: Animaux / Humains / Mâle langue: Chinois Texte intégral: China Journal of Chinese Materia Medica Année: 2015 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Sujet Principal: Médicaments issus de plantes chinoises / Chimie / Interleukine-6 / Facteur de nécrose tumorale alpha / Rat Sprague-Dawley / Stress oxydatif / Grifola / Traitement médicamenteux / Stéatose hépatique non alcoolique / Foie Type d'étude: Étude pronostique Limites du sujet: Animaux / Humains / Mâle langue: Chinois Texte intégral: China Journal of Chinese Materia Medica Année: 2015 Type: Article