Simultaneous determination of five sesquiterpene lactones in Carpesium abrotanoides by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1783-1786, 2015.
Article
de Zh
| WPRIM
| ID: wpr-351265
Bibliothèque responsable:
WPRO
ABSTRACT
An HPLC method was established to simultaneously determine the five sesquiterpene lactones, carabrone, carabrol, 2-desoxy-4-epi-pulchellin, telekinand 11(13)-dehydroivaxillin in Carpesium abrotanoides. Samples were analyzed on a kromasil C18 column (4.6 mm x 250 mm, 5 µm); mobile phase: A was acetonitrile, B was water, with gradient elution; flow speed: 1.0 mL · min(-1); detection was carried out using a photodiode array detector at 211 nm; temperatureof column: 30 °C. The five sesquiterpene lactones were well separated with good linear correlations in the range of 0.270-2.700 (r = 0.999 7 ), 1.336-13.360 (r = 0.999 6), 0.258-2.580 (r = 0.999 7), 0.238-2.380 (r = 0.999 9), 0.490-4.900 µg (r = 0.999 9) for carabrone, carabrol, 2-desoxy-4-epi-pulchellin, telekin and 11 (13) -dehydroivaxillin. The average recoveries of these five sesquiterpene lactoneswere 96.78%-98.41% (RSD 1.3%-2.9%). The method was simple, repeatable and stable, which could be used for quality control of C. abrotanoide.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Sesquiterpènes
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Médicaments issus de plantes chinoises
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Chimie
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Chromatographie en phase liquide à haute performance
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Asteraceae
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Lactones
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Méthodes
langue:
Zh
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2015
Type:
Article