Determination of two polyacetylenes in Herba Lobeliae Chinensis by HPLC analysis / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 744-746, 2006.
Article
de Zh
| WPRIM
| ID: wpr-351766
Bibliothèque responsable:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To develop an HPLC method for determination of two polyacetylenes, lobetyolin and lobetyolinin, in Herba Lobeliae Chinensis.</p><p><b>METHOD</b>C18 column was used with the mobile phase consisted of acetonitrile and water. Linear gradient elution from 10% to 40% acetonitrile in 25 min was applied, at the flow rate of 1.0 mL x min(-1), the detection wavelength was at 267 nm.</p><p><b>RESULT</b>Lower contents of lobetyolin and lobtyolinin were found in collected samples of Herba Lobeliae Chinensis. The highest amounts of lobetyolin and lobetyolinin were found to be 0.461 and 0.436 mg x g(-1) in a sample procured from Hong Kong. However, there were no lobetyolin and lobetyolinin in some of the samples.</p><p><b>CONCLUSION</b>A simple and effective HPLC method to analyze the two polyacetylenes in Herba Lobeliae Chinensis was established. It could be applied for the quality control of this herb.</p>
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Plantes médicinales
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Contrôle de qualité
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Structure moléculaire
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Chimie
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Reproductibilité des résultats
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Chromatographie en phase liquide à haute performance
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Lobelia
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Polymère de polyacétylène
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Méthodes
langue:
Zh
Texte intégral:
China Journal of Chinese Materia Medica
Année:
2006
Type:
Article