Protective effects of Liuweidihuangwan on hepatic insulin resistance in OLETF rats / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1824-1827, 2012.
Article
Dans Chinois
| WPRIM
| ID: wpr-352325
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the mechanism through which Liuweidihuangwan improves hepatic insulin resistance in type 2 diabetic rats.</p><p><b>METHODS</b>With LETO rats as the normal control group, OLETF rats were treated daily with or without Liuweidihuangwan. At 8, 32, and 40 weeks of the treatment, 3 rats were randomly selected from each group for histological examination of the liver tissues and for detection of phosphoenolpyruvate carboxylase kinase (PEPCK) mRNA expression using RT-PCR and insulin receptor substrate-1 (IRS-1) and IRS-2 protein expressions using Western blotting.</p><p><b>RESULTS</b>Compared with LETO rats, OLETF rats showed progressive destruction of the lobular structures and hepatic steatosis in the liver over time. OLETF rats with Liuweidihuangwan treatment had basically normal lobular structure with only mild fatty degeneration in the liver. RT-PCR detection demonstrated a significantly higher PEPCK mRNA expression in untreated OLETF rats than in LETO rats (P<0.01), but a significantly lowered PEPCK expression in OLETF rats after Liuweidihuangwan dosing (P<0.01). Western blotting showed that significantly lower p-IRS-1 and p-IRS-2 protein expressions in untreated OLETF rats than those in LETO rats and treated OLTEF rats (P<0.05).</p><p><b>CONCLUSION</b>Liuweidihuangwan improves hepatic insulin resistance in OLETF rats by inhibiting the activity of gluconeogenic key enzyme (PEPCK) in the liver and enhancing IRS-1 and IRS-2 expressions in the insulin signaling pathway.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Anatomopathologie
/
Pharmacologie
/
Phosphoenolpyruvate carboxykinase (GTP)
/
Insulinorésistance
/
Médicaments issus de plantes chinoises
/
Transduction du signal
/
Rats de lignée OLETF
/
Utilisations thérapeutiques
/
Protéines et peptides de signalisation intracellulaire
/
Diabète expérimental
Limites du sujet:
Animaux
langue:
Chinois
Texte intégral:
Journal of Southern Medical University
Année:
2012
Type:
Article
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