Protein profiles of serum in gastric cancer at a high-risk area by SELDI-TOF mass spectrometry / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 677-680, 2008.
Article
Dans Chinois
| WPRIM
| ID: wpr-352413
ABSTRACT
<p><b>OBJECTIVE</b>To explore the specific and sensitive biomarkers for gastric cancer detection, a surface-enhanced laser desorption and ionization protein chip mass spectrometry (SELDI-TOF-MS) was used to generate protein profiles of serum in gastric cancer at a high-risk area.</p><p><b>METHODS</b>A total of 36 gastric cancer cases and 46 subjects with superficial gastritis were selected from Linqu county, Shandong province, a high-risk area of gastric cancer. Serum samples were collected and Q10 protein chips were used to detect the serum proteomic patterns, and the sensitivity and specificity were assessed.</p><p><b>RESULTS</b>For the comparison of the gastric cancer group (26 out of 36 gastric cancer cases) versus superficial gastritis group (37 out of 46 subjects), the 6 most discriminating peaks (m/z 8587, 6945, 8243, 3899, 7035, and 9943) were identified by the ProteinChip Data Analysis System (ZUCIPDAS). The sensitivity and specificity of this pattern were 88.5% and 97.3%, respectively. A total of 19 subjects (10 gastric cancer cases and 9 superficial gastritis subjects) was selected to test the accuracy of this pattern by using blind method, and the sensitivity and specificity were 80.0% and 88.9% ,respectively.</p><p><b>CONCLUSION</b>Our findings suggest that SELDI profiling of serum might be a potential for gastric cancer detection and screening in high-risk population.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Spectrométrie de masse
/
Tumeurs de l'estomac
/
Sang
/
Cartographie peptidique
/
Protéines du sang
/
Marqueurs biologiques tumoraux
/
Études cas-témoins
/
Chine
/
Épidémiologie
/
Études de cohortes
Type d'étude:
Etude diagnostique
/
Etude d'étiologie
/
Etude d'incidence
/
Étude observationnelle
/
Étude pronostique
/
Facteurs de risque
Limites du sujet:
Adulte très âgé
/
Femelle
/
Humains
/
Mâle
Pays comme sujet:
Asie
langue:
Chinois
Texte intégral:
Chinese Journal of Preventive Medicine
Année:
2008
Type:
Article
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