Expression of thrombin and its associated protein in cerebellum of human and rat after intracerebral hemorrhage / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 2077-2081, 2010.
Article
Dans Anglais
| WPRIM
| ID: wpr-352509
ABSTRACT
<p><b>BACKGROUND</b>Intracerebral hemorrhage (ICH) can cause brain damage through a number of pathways. The purpose of the study was to explore the effect of thrombin, protease nexin-1 (PN-1) and protease activated receptor-1 (PAR-1) in rat and human cerebellum after ICH.</p><p><b>METHODS</b>A model of ICH was produced in adult Sprague-Dawley rats by direct injection of autologous blood (50 microl) into caudate nucleus. Patients with injured hemorrhage were also enrolled in this study. Different expressions of thrombin, PAR-1, PN-1 were detected in rat and human cerebellum by immunohistochemistry and in situ hybridization.</p><p><b>RESULTS</b>In rat cerebellum, thrombin protein significantly increased at 6 hours and reached the maximum 2 days after ICH. The expression of PAR-1 protein reached the maximum at 24 - 48 hours, and then began to decrease. The expression of PN-1 protein reached the maximum at 3 hours, decreased somewhat after that and increased a little at 5 days after ICH. While in human cerebellum, the changing tendency of thrombin, PAR-1 and PN-1 was almost conform to the rat.</p><p><b>CONCLUSION</b>In cerebellum, thrombin can activate PAR-1 expression after ICH, and PN-1 appears quickly after ICH in order to control the deleterious effect of thrombin.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Immunohistochimie
/
Thrombine
/
Cervelet
/
Hémorragie cérébrale
/
Rat Sprague-Dawley
/
Hybridation in situ
/
Récepteur de type PAR-1
/
Serpine E2
/
Génétique
/
Métabolisme
Limites du sujet:
Aged80
/
Animaux
/
Femelle
/
Humains
/
Mâle
langue:
Anglais
Texte intégral:
Chinese Medical Journal
Année:
2010
Type:
Article
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