Stable transfection of estrogen receptor-alpha suppresses expression of cyclooxygenase-2 and vascular endothelial growth factor-C in MDA-MB-231 breast cancer cells / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1989-1994, 2010.
Article
Dans Anglais
| WPRIM
| ID: wpr-352524
ABSTRACT
<p><b>BACKGROUND</b>Estrogen receptor (ER)-negative breast cancer cells are more aggressive than ER-positive cells. Elevated levels of cyclooxygenase-2 (COX-2) and vascular endothelial growth factor-C (VEGF-C) expression have been detected in cultured human breast cancer cells and are associated with negative hormone receptor status. In this study, we created ERalpha stable transfectants in MDA-MB-231 cells to explore the effect of ERalpha on cell growth and COX-2 and VEGF-C expression.</p><p><b>METHODS</b>The green fluorescent protein (GFP)-ERalpha plasmids were stably transfected into ER-negative MDA-MB-231 cells. The proliferation and migration of untransfected MDA-MB-231 cells, ERalpha-transfected MDA-MB-231 cells and ER-positive MCF-7 cells were determined. The expression of COX-2, and the levels of VEGF-C mRNA and the VEGF-C secretion concentration were assayed in these cell lines.</p><p><b>RESULTS</b>The proliferation and migration capacities of ERalpha-tranfected MDA-MB-231 cells were significantly decreased (P < 0.05). The expression of COX-2 was significantly lower in ERalpha-tranfected MDA-MB-231 cells than in untranfected MDA-MB-231 cells. The mRNA and protein levels of VEGF-C were lower in ERalpha-tranfected MDA-MB-231 cells than in untransfected MDA-MB-231 cells (P < 0.05).</p><p><b>CONCLUSIONS</b>ERalpha stable transfection inhibits proliferation and migration capacities of MDA-MB-231 cells and decreases expression of COX-2 and VEGF-C. The decreases of proliferation and migration capacities may be related to suppression of COX-2 and VEGF-C expression.</p>
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Physiologie
/
Tumeurs du sein
/
Test ELISA
/
Transfection
/
Cycle cellulaire
/
Mouvement cellulaire
/
Technique de Western
/
Réaction de polymérisation en chaîne
/
Lignée cellulaire tumorale
/
Facteur de croissance endothéliale vasculaire de type C
Limites du sujet:
Femelle
/
Humains
langue:
Anglais
Texte intégral:
Chinese Medical Journal
Année:
2010
Type:
Article
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