Electrophysiological effects of resveratrol on guinea pig papillary muscles / 生理学报
Acta Physiologica Sinica
; (6): 708-712, 2004.
Article
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| ID: wpr-352710
Responsable en Bibliothèque :
WPRO
ABSTRACT
The purpose of this study was to investigate the electrophysiological effects of resveratrol on guinea pig papillary muscles and the underlying mechanism. Action potentials were recorded by using intracellular microelectrode technique. The results obtained are as follows: (1) In normal papillary muscles, resveratrol (30, 60, and 120 micromol/L) shortened the duration of action potential (APD) in a concentration-dependent manner. (2) In partially depolarized papillary muscles, resveratrol (60 micromol/L ) not only shortened APD, but also decreased the amplitude of action potential (APA), overshoot (OS) and maximal rate of depolarization in phase 0 (Vmax). (3) Perfusion with Ca2+-free K-H solution, completely abolished the effects of resveratrol (60 micromol/L) on papillary muscles. (4) Application of potassium channel blocker tetraethylammonium chloride (TEA, 20 mmol/L) did not prevent the effect of resveratrol (60 micromol/L) on action potential. (5) Pretreatment with NG-nitro-L-arginine methyl ester (L-NAME, 1 mmol/L), a nitric oxide (NO) synthase inhibitor, failed to abolish the effect of resveratrol (60 micromol/L). All these results indicate that the electrophysiological effects of resveratrol on guinea pig papillary muscles are likely due to the reduction of calcium influx, which might not be mediated by NO.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Muscles papillaires
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Pharmacologie
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Physiologie
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Stilbènes
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Techniques in vitro
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Potentiels d'action
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Inhibiteurs des canaux calciques
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Cochons d'Inde
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Microélectrodes
Limites du sujet:
Animals
langue:
En
Texte intégral:
Acta Physiologica Sinica
Année:
2004
Type:
Article