Activation of chloride current and decrease of cell volume by ATP in nasopharyngeal carcinoma cells / 生理学报
Acta Physiologica Sinica
; (6): 691-696, 2004.
Article
de En
| WPRIM
| ID: wpr-352713
Bibliothèque responsable:
WPRO
ABSTRACT
Whole-cell patch clamp and cell volume measurement techniques were used to investigate the ATP-activated chloride current and the ATP effect on cell volume in nasopharyngeal carcinoma cells. Extracellular application of ATP in micromolar concentrations activated a current with the properties of modest outward rectification and negligible time-dependent inactivation in a dose-dependent manner. The current reversed at a potential [(-0.05+/-0.03) mV] close to the Cl- equilibrium potential (-0.9 mV). Substitution of Cl- with gluconate in the extracellular solution decreased the ATP-activated current and shifted the reversal potential positively. NPPB, one of the chloride channel blockers, inhibited the current by (81.03+/-9.36)%. The current was also depressed by the P2Y purinoceptor antagonist, reactive blue 2, by (67.39+/-5.06)%. ATP (50 micromol/L) decreased the cell volume under the isotonic condition. Depletion of extracellular and intracellular Cl- abolished the ATP effect on cell volume. The results suggest that extracellular ATP of micromolar scales can induce a chloride current associated with cell volume regulation by activation of chloride channel through binding to purinoceptor P2Y.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Anatomopathologie
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Pharmacologie
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Physiologie
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Cellules cancéreuses en culture
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Adénosine triphosphate
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Tumeurs du rhinopharynx
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Canaux chlorure
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Techniques de patch-clamp
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Taille de la cellule
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Métabolisme
Limites du sujet:
Humans
langue:
En
Texte intégral:
Acta Physiologica Sinica
Année:
2004
Type:
Article